Gene Expression

Novel Technology for Gene Quantification

• Quantitative method based on reverse transcription PCR (RT-PCR)
• Patented universal primer strategy for high throughput multiplexing
• Homogenous single tube assays

Taking Gene Expression to the Next Level

• Patented universal primer strategy for high throughput multiplexing
• Low sample volume (25ng of total RNA) - up to 60 genes per reaction
• Fast turnaround designing and qualifying new multiplex assays.

Part of a Complete Gene Expression Solution

• Integrates into a comprehensive gene expression service
• TaqMan and Affymetrix
• Can be performed under GLP and CLIA conditions at AltheaDx
• Assays can be transferred to client lab, using Beckman GeXP systems.

RNA samples were derived from fresh frozen prostate tumor tissue and from a 16 year-old FFPE tissue block. Ten reference genes were quantified using single multiplex XP-PCR reactions and real-time PCR. Even in the degraded fixed tissue sample (top right), XP-PCR generated good signal outperforming real-time PCR (lower right).