Expertise with Fixed Tissue Analysis

AltheaDx has developed a proven strategy for processing formalin-fixed, paraffin embedded (FFPE) tissue samples.

Existing banked and well-characterized tissue collections are a treasure chest of genetic information; however, DNA and RNA from FFPE tissues is highly degraded. AltheaDx’s methods can extract highly valuable information from these samples.

AltheaDx provides a range of Short Fragment Assay (SFA) formats for the analysis of degraded samples, including FFPE tissue blocks. We have extensive experience in high-throughput sample analysis using our propriety XP™- PCR technology to monitor gene expression in highly degraded RNA samples.

Our testing facilities have been specifically designed for maximum regulatory compliance and include separate suites dedicated to sample receiving, RNA extraction, sample preparation, and gene expression analysis available under GLP conditions. Our in-depth technical and regulatory expertise, state-of-the-art equipment and technology, and strict compliance to regulatory guidelines allow Althea to provide the highest quality, validated analytical services with rapid turnaround.

Sample AltheaDx Results: Multiplexed XP-PCR from FFPE Tissues

RNA samples were derived from fresh frozen prostate tumor tissue and from a 16 year-old FFPE tissue block. Ten reference genes were quantified using single multiplex XP-PCR reactions and real-time PCR. Even in the degraded fixed tissue sample (top right), XP-PCR generated good signal outperforming real-time PCR (lower right).