AltheaDx to Present at AACR Annual Meeting 2013
AACR Annual Meeting, Washington D.C. April 6-10, 2013
“Targeted RNA Sequencing For Expression Analysis of Breast Cancer Patient Samples”
Session ID: Molecular and Cellular Biology 51
Session Date and Time: Tuesday, April 9th, 2013
Time: 1:00 PM-5:00 PM
Location: Hall A-C, Poster Session 28
Background: Gene expression profiling has been shown to be effective in the analysis of postoperative tumor samples in various cancers. However, for studies requiring the analysis of small specimens, such as core biopsies, the limited amount of available material makes multi-gene analyses exceedingly difficult. Moreover, microarray based analyses provide somewhat limited dynamic range and multi-gene qRT-PCR analyses often require in excess of 100 ng of input RNA per sample. To this end, we describe the development of targeted RNA sequencing methodology which combines the power of a universal RNA amplification with NGS for ultra-deep expression analysis of multiple target genes, enabling <100 ng of sample input for multi-gene analysis in a single tube format.